Journal: Journal of Animal Science

Article Title: The role of the y(+)L-type amino acid transporter 2 in promoting zinc absorption as zinc proteinate with moderate chelation strength in broiler primary duodenal epithelial cells

doi: 10.1093/jas/skaf466

Figure Lengend Snippet: Effects of different siRNAs transfections on y+LAT2 mRNA expression levels in BPDECs (experiment 1). The y+LAT2 mRNA expression levels were calculated as the relative quantities (RQ) of the y+LAT2 mRNA to the geometric mean of β-actin and GAPDH mRNA using the 2 −ΔΔCT method. BPDECs: broiler primary duodenal epithelial cells. Different letters indicated significant differences among treatments ( P < 0.05). Data are means ± SE ( n = 5 to 6).

Article Snippet: After the end of the transfer, the membrane was blocked using 5% BSA for 1 h, cleaned with Tris-buffered saline containing 0.02% (v/v) Tween-20 (TBST) for 3 times, and then incubated with primary antibodies against y+LAT2 (Proteintech, China) or β-tubulin (Huaxingbio, China) overnight at 4 °C.

Techniques: Transfection, Expressing

Journal: Journal of Animal Science

Article Title: The role of the y(+)L-type amino acid transporter 2 in promoting zinc absorption as zinc proteinate with moderate chelation strength in broiler primary duodenal epithelial cells

doi: 10.1093/jas/skaf466

Figure Lengend Snippet: Effect of the y+LAT2 silencing on Zn absorption and y+LAT2 expression levels in BPDECs under different Zn sources (experiment 2). (A) The mRNA expression levels of y+LAT2 . (B) The protein expression levels of y+LAT2. C The amount of Zn absorption. The y+LAT2 mRNA expression levels were calculated as the relative quantities (RQ) of the y+LAT2 mRNA to the geometric mean of β-actin and GAPDH mRNA using the 2 −ΔΔCT method. The y+LAT2 protein expression levels were calculated as the relative quantities (RQ) of the y+LAT2 protein band intensity to the β-tubulin protein band intensity. ZnSO 4 : Zn sulfate. Zn-Prot M: Zn proteinate with moderate chelation strength (Q f = 51.6). BPDECs: broiler primary duodenal epithelial cells. Different letters indicated significant differences among treatments ( P < 0.05). Data are means ± SE ( n = 5 to 6).

Article Snippet: After the end of the transfer, the membrane was blocked using 5% BSA for 1 h, cleaned with Tris-buffered saline containing 0.02% (v/v) Tween-20 (TBST) for 3 times, and then incubated with primary antibodies against y+LAT2 (Proteintech, China) or β-tubulin (Huaxingbio, China) overnight at 4 °C.

Techniques: Expressing

Journal: Journal of Animal Science

Article Title: The role of the y(+)L-type amino acid transporter 2 in promoting zinc absorption as zinc proteinate with moderate chelation strength in broiler primary duodenal epithelial cells

doi: 10.1093/jas/skaf466

Figure Lengend Snippet: Effect of the y+LAT2 overexpression on Zn absorption and y+LAT2 expression levels in BPDECs under different Zn sources (experiment 3). (A) The mRNA expression levels of y+LAT2 . (B) The protein expression levels of y+LAT2. C The amount of Zn absorption. The y+LAT2 mRNA expression levels were calculated as the relative quantities (RQ) of the y+LAT2 mRNA to the geometric mean of β-actin and GAPDH mRNA using the 2 −ΔΔCT method. The y+LAT2 protein expression levels were calculated as the relative quantities (RQ) of the y+LAT2 protein band intensity to the β-tubulin protein band intensity. ZnSO 4 : Zn sulfate. Zn-Prot M: Zn proteinate with moderate chelation strength (Q f = 51.6). BPDECs: broiler primary duodenal epithelial cells. Different letters indicated significant differences among treatments ( P < 0.05). Data are means ± SE ( n = 5 to 6).

Article Snippet: After the end of the transfer, the membrane was blocked using 5% BSA for 1 h, cleaned with Tris-buffered saline containing 0.02% (v/v) Tween-20 (TBST) for 3 times, and then incubated with primary antibodies against y+LAT2 (Proteintech, China) or β-tubulin (Huaxingbio, China) overnight at 4 °C.

Techniques: Over Expression, Expressing

Journal:

Article Title: In vivo tracking and protective properties of Yersinia -specific intestinal T cells

doi: 10.1046/j.1365-2249.1998.00659.x

Figure Lengend Snippet: Adoptive transfer of PKH2-GL-labelled or non-labelled Yersinia-specific T cell lines (a) GLP 2.92 and (b) LK 5.93 1 day before orogastric infection with 7 × 108Yersinia enterocolitica. Five days after infection, mice were killed and bacterial counts in Peyer's patches (PP), mesenteric lymph nodes (MLN) and spleen determined as described in Materials and Methods. The values given are the means of 10 mice per group. *Values that differ from the control group at P < 0.05.

Article Snippet: Two weeks after restimulation T cells were purified and labelled with the green fluorescent cell linker kit PKH2-GL (Dianova; 2 × 10 −6 m ) according to the instructions of the manufacturers based on the data by Horan et al . [ 33 ].

Techniques: Adoptive Transfer Assay, Infection

Journal:

Article Title: In vivo tracking and protective properties of Yersinia -specific intestinal T cells

doi: 10.1046/j.1365-2249.1998.00659.x

Figure Lengend Snippet: Proliferative responses of PKH2-GL-labelled and non-labelled T cells (GLP 2.92) 24, 48 and 72 h after stimulation with concanavalin A (Con A). Proliferation of cells was determined by 3H-thymidine uptake as described in Materials and Methods. *Values that differ from the non-labelled control cells at P < 0.05. ▪, Control cells; □, PKH2-GL-labelled cells.

Article Snippet: Two weeks after restimulation T cells were purified and labelled with the green fluorescent cell linker kit PKH2-GL (Dianova; 2 × 10 −6 m ) according to the instructions of the manufacturers based on the data by Horan et al . [ 33 ].

Techniques:

Journal:

Article Title: In vivo tracking and protective properties of Yersinia -specific intestinal T cells

doi: 10.1046/j.1365-2249.1998.00659.x

Figure Lengend Snippet: IFN-γ production of PKH2-GL-labelled and non-labelled T cells (GLP 2.92) 24, 48 and 72 h after stimulation with concanavalin A (Con A). IFN-γ production was determined in cell culture supernatants by capture ELISA as described in Materials and Methods. *Values that differ from the non-labelled control cells at P < 0.05. ▪, Control cells; □, PKH2-GL-labelled cells.

Article Snippet: Two weeks after restimulation T cells were purified and labelled with the green fluorescent cell linker kit PKH2-GL (Dianova; 2 × 10 −6 m ) according to the instructions of the manufacturers based on the data by Horan et al . [ 33 ].

Techniques: Cell Culture, Enzyme-linked Immunosorbent Assay

Journal:

Article Title: In vivo tracking and protective properties of Yersinia -specific intestinal T cells

doi: 10.1046/j.1365-2249.1998.00659.x

Figure Lengend Snippet: In vivo tracking of adoptively transferred PKH2-GL-labelled T cells after Yersinia enterocolitica infection*

Article Snippet: Two weeks after restimulation T cells were purified and labelled with the green fluorescent cell linker kit PKH2-GL (Dianova; 2 × 10 −6 m ) according to the instructions of the manufacturers based on the data by Horan et al . [ 33 ].

Techniques: In Vivo, Infection

Journal:

Article Title: In vivo tracking and protective properties of Yersinia -specific intestinal T cells

doi: 10.1046/j.1365-2249.1998.00659.x

Figure Lengend Snippet: Tracking of PKH2-GL-labelled T cells (GLP 2.92) by FACS analysis in spleen 4 days after adoptive transfer of 2 × 107 GLP 2.92 T cells. Fifty thousand cells per sample were analysed. Non-labelled T cells (GLP 2.92, left), PKH2-GL-labelled T cells (GLP 2.92, middle) and recovered PKH2-GL T cells (GLP 2.92, spleen, right). Ordinate, number of cells; abscissa, relative fluorescence signal.

Article Snippet: Two weeks after restimulation T cells were purified and labelled with the green fluorescent cell linker kit PKH2-GL (Dianova; 2 × 10 −6 m ) according to the instructions of the manufacturers based on the data by Horan et al . [ 33 ].

Techniques: Adoptive Transfer Assay, Fluorescence

Journal:

Article Title: In vivo tracking and protective properties of Yersinia -specific intestinal T cells

doi: 10.1046/j.1365-2249.1998.00659.x

Figure Lengend Snippet: Double staining of PKH2-GL-labelled GLP 2.92 T cells recovered from the lung 4 days after adoptive transfer. The ordinate indicates the relative fluorescence intensity for CD4, the abscissa indicates the relative fluorescence intensity of PKH2-GL-labelled T cells.

Article Snippet: Two weeks after restimulation T cells were purified and labelled with the green fluorescent cell linker kit PKH2-GL (Dianova; 2 × 10 −6 m ) according to the instructions of the manufacturers based on the data by Horan et al . [ 33 ].

Techniques: Double Staining, Adoptive Transfer Assay, Fluorescence

Journal:

Article Title: In vivo tracking and protective properties of Yersinia -specific intestinal T cells

doi: 10.1046/j.1365-2249.1998.00659.x

Figure Lengend Snippet: Fluorescence microscopy of PKH2-GL-labelled T cells 1 day (a) and 4 days (b) after staining with PKH2-GL and culture in vitro. Cryosections of spleen (c) and mesenteric lymph nodes (MLN) (e) 4 days after transfer of 2 × 107 PKH2-GL-labelled LK 5.93 T cells and cryosections of spleen (d) and MLN (f) 4 days after a transfer of 2 × 107 PKH2-GL-labelled GLP 2.92 T cells.

Article Snippet: Two weeks after restimulation T cells were purified and labelled with the green fluorescent cell linker kit PKH2-GL (Dianova; 2 × 10 −6 m ) according to the instructions of the manufacturers based on the data by Horan et al . [ 33 ].

Techniques: Fluorescence, Microscopy, Staining, In Vitro

Journal: PLoS ONE

Article Title: Cyclooxgenase-2 Inhibiting Perfluoropoly (Ethylene Glycol) Ether Theranostic Nanoemulsions— In Vitro Study

doi: 10.1371/journal.pone.0055802

Figure Lengend Snippet: Composition of nanoemulsions.

Article Snippet: CellVue® NIR815 (786 nm/814 nm) and CellVue® Burgundy (683 nm/707 nm) Fluorescent Cell Linker Kit was purchased from Molecular Targeting Technologies, Inc. (MTTI), West Chester, PA, USA.

Techniques:

Journal: ACS Infectious Diseases

Article Title: Cowpea Mosaic Virus Nanoparticle Vaccine Candidates Displaying Peptide Epitopes Can Neutralize the Severe Acute Respiratory Syndrome Coronavirus

doi: 10.1021/acsinfecdis.1c00410

Figure Lengend Snippet: SARS-CoV-2 S protein domain map. Subunits and domains are not shown to scale. The positions of B-cell epitopes are shown, and the color code matches Table . SP = signal peptide; NTD = N-terminal domain; RBD = receptor-binding domain; FP = fusion peptide; HR1 = heptad repeat 1; HR2 = heptad repeat 2; TM = transmembrane domain; CP = cytoplasmic domain.

Article Snippet: The five B-cell epitopes appended with an N-terminal cysteine residue a and triple glycine (GGG) linker were purchased from GenScript, with the following peptide sequences: 317 = C-GGG-KGIYQTSN, 362 = C-GGG-ATRFASVYAWNRKRISN, 988 = C-GGG-AISSVLNDILSRLDKVE, 1173 = C-GGG-KNHTSPDVDLGDISGIN, and 1209 = C-GGG-EIDRLNEVAKNLNESLIDLQEL.

Techniques: Binding Assay

Journal: ACS Infectious Diseases

Article Title: Cowpea Mosaic Virus Nanoparticle Vaccine Candidates Displaying Peptide Epitopes Can Neutralize the Severe Acute Respiratory Syndrome Coronavirus

doi: 10.1021/acsinfecdis.1c00410

Figure Lengend Snippet: B-Cell Epitopes from the SARS-CoV-2/SARS-CoV S Proteins

Article Snippet: The five B-cell epitopes appended with an N-terminal cysteine residue a and triple glycine (GGG) linker were purchased from GenScript, with the following peptide sequences: 317 = C-GGG-KGIYQTSN, 362 = C-GGG-ATRFASVYAWNRKRISN, 988 = C-GGG-AISSVLNDILSRLDKVE, 1173 = C-GGG-KNHTSPDVDLGDISGIN, and 1209 = C-GGG-EIDRLNEVAKNLNESLIDLQEL.

Techniques: Sequencing, Solubility